cas9 grna Search Results


custom alt r crispr cas9 guide rna  (Integrated DNA Technologies)
95
Integrated DNA Technologies custom alt r crispr cas9 guide rna
DNA-PKcs inhibition augments the efficiency of HDR-mediated gene editing Homozygous tagging of the centriolar distal appendage protein SCLT1 with the V5-epitope is presented as example. Characterization of the polyclonal population of RPE1 cells resulting from the electroporation with a SCLT1-V5-tagging RNP mixture <t>(Cas9,</t> SCLT1 gRNA and SCLT1-V5 Ultramer®) in the absence (DMSO) or presence (NU7441) of the DNA-PKcs inhibitor. (A) Centrioles are stained with anti-γTub antibody, V5 signal (if present) co-localizes with one of the two γTub-positive centrioles. White arrows indicate V5+ cells. Scale bar, 10 μm. Blow-ups without Hoechst 33342 are magnified 2.5×. (B) Bar chart showing the percentage of V5+ cells for each condition. N=3 independent experiments (with >100 cells visually scored for each condition), unpaired Student’s t test, ∗∗ = p < 0.05. (C) Electroporated RPE1, both untreated (DMSO) and treated with NU7441 were probed for V5 and compared to RPE1 parental cells. V5 signal, when present, corresponds to the expected molecular weight of 75 kDa.
Custom Alt R Crispr Cas9 Guide Rna, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/CRISPR-Cas9+gRNA/pmc08025146-30-1-0
Average 95 stars, based on 1 article reviews
custom alt r crispr cas9 guide rna - by Bioz Stars, 2026-11
95/100 stars
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lenti cas9 grna gfp vector  (Addgene inc)
93
Addgene inc lenti cas9 grna gfp vector
DNA-PKcs inhibition augments the efficiency of HDR-mediated gene editing Homozygous tagging of the centriolar distal appendage protein SCLT1 with the V5-epitope is presented as example. Characterization of the polyclonal population of RPE1 cells resulting from the electroporation with a SCLT1-V5-tagging RNP mixture <t>(Cas9,</t> SCLT1 gRNA and SCLT1-V5 Ultramer®) in the absence (DMSO) or presence (NU7441) of the DNA-PKcs inhibitor. (A) Centrioles are stained with anti-γTub antibody, V5 signal (if present) co-localizes with one of the two γTub-positive centrioles. White arrows indicate V5+ cells. Scale bar, 10 μm. Blow-ups without Hoechst 33342 are magnified 2.5×. (B) Bar chart showing the percentage of V5+ cells for each condition. N=3 independent experiments (with >100 cells visually scored for each condition), unpaired Student’s t test, ∗∗ = p < 0.05. (C) Electroporated RPE1, both untreated (DMSO) and treated with NU7441 were probed for V5 and compared to RPE1 parental cells. V5 signal, when present, corresponds to the expected molecular weight of 75 kDa.
Lenti Cas9 Grna Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/Lenti-Cas9-gRNA-GFP+(Plasmid+%23124770)/pmc07354079-1043-6-8
Average 93 stars, based on 1 article reviews
lenti cas9 grna gfp vector - by Bioz Stars, 2026-11
93/100 stars
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cas9 lentivirus vector  (Cyagen Biosciences)
90
Cyagen Biosciences cas9 lentivirus vector
Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with <t>Cas9</t> and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.
Cas9 Lentivirus Vector, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/Lentivirus+(single+gRNA)%3B+with+Cas9/pmc11197767-87-1-11
Average 90 stars, based on 1 article reviews
cas9 lentivirus vector - by Bioz Stars, 2026-11
90/100 stars
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gfp lenti cas9 grna tagbfp2 124774 co express cas9  (Addgene inc)
94
Addgene inc gfp lenti cas9 grna tagbfp2 124774 co express cas9
Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with <t>Cas9</t> and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.
Gfp Lenti Cas9 Grna Tagbfp2 124774 Co Express Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/Lenti-Cas9-gRNA-TagBFP2+(Plasmid+%23124774)/pmc06741428-529-31-44
Average 94 stars, based on 1 article reviews
gfp lenti cas9 grna tagbfp2 124774 co express cas9 - by Bioz Stars, 2026-11
94/100 stars
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harvard medical school  (Addgene inc)
93
Addgene inc harvard medical school
Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with <t>Cas9</t> and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.
Harvard Medical School, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/MiniCoopR+2xU6%3AgRNA%2C+mitfa%3ACas9+(Plasmid+%23118844)/pmc10551668-240-13-18
Average 93 stars, based on 1 article reviews
harvard medical school - by Bioz Stars, 2026-11
93/100 stars
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cas9 sgrna plasmid p426 cas9 grna ars1114a  (Addgene inc)
94
Addgene inc cas9 sgrna plasmid p426 cas9 grna ars1114a
Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with <t>Cas9</t> and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.
Cas9 Sgrna Plasmid P426 Cas9 Grna Ars1114a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/p426_Cas9_gRNA-ARS1114a+(Plasmid+%2387397)/bio_rxiv__64898__2026__03__29__715152-188-20-23
Average 94 stars, based on 1 article reviews
cas9 sgrna plasmid p426 cas9 grna ars1114a - by Bioz Stars, 2026-11
94/100 stars
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grna cas9 plasmids  (Cyagen Biosciences)
92
Cyagen Biosciences grna cas9 plasmids
Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with <t>Cas9</t> and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.
Grna Cas9 Plasmids, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/Regular+plasmid+(single+gRNA)%3B+with+Cas9/pmc06376184-87-12-17
Average 92 stars, based on 1 article reviews
grna cas9 plasmids - by Bioz Stars, 2026-11
92/100 stars
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p426 cas9 grna ars511b  (Addgene inc)
93
Addgene inc p426 cas9 grna ars511b
Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with <t>Cas9</t> and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.
P426 Cas9 Grna Ars511b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/p426_Cas9_gRNA-ARS511b+(Plasmid+%2387387)/pmc09680028-203-12-13
Average 93 stars, based on 1 article reviews
p426 cas9 grna ars511b - by Bioz Stars, 2026-11
93/100 stars
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pzdonor nm cas9 grna hu6  (Addgene inc)
90
Addgene inc pzdonor nm cas9 grna hu6
Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with <t>Cas9</t> and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.
Pzdonor Nm Cas9 Grna Hu6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/pZDonor-Nm-Cas9-gRNA-hU6+(Plasmid+%2361366)/pmc04430400-135-16-17
Average 90 stars, based on 1 article reviews
pzdonor nm cas9 grna hu6 - by Bioz Stars, 2026-11
90/100 stars
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px458 cas9 gfp  (Addgene inc)
92
Addgene inc px458 cas9 gfp
Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with <t>Cas9</t> and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.
Px458 Cas9 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/pX458-Ef1a-Cas9-GFP+(gRNA%3A+DHS-4D-2)+(Plasmid+%23159663)/pm38431841-195-5-9
Average 92 stars, based on 1 article reviews
px458 cas9 gfp - by Bioz Stars, 2026-11
92/100 stars
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cas9 plasmids  (Cyagen Biosciences)
93
Cyagen Biosciences cas9 plasmids
Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with <t>Cas9</t> and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.
Cas9 Plasmids, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/Regular+plasmid+(single+gRNA)%3B+without+Cas9/pmc09293912-234-5-34
Average 93 stars, based on 1 article reviews
cas9 plasmids - by Bioz Stars, 2026-11
93/100 stars
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px458 vector encoding cas9 nuclease  (Addgene inc)
92
Addgene inc px458 vector encoding cas9 nuclease
Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with <t>Cas9</t> and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.
Px458 Vector Encoding Cas9 Nuclease, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+grna/pX458-Ef1a-Cas9-H2B-GFP+(gRNA%3A+Ascl1-1)+(Plasmid+%23159656)/pmc06402561-402-16-25
Average 92 stars, based on 1 article reviews
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DNA-PKcs inhibition augments the efficiency of HDR-mediated gene editing Homozygous tagging of the centriolar distal appendage protein SCLT1 with the V5-epitope is presented as example. Characterization of the polyclonal population of RPE1 cells resulting from the electroporation with a SCLT1-V5-tagging RNP mixture (Cas9, SCLT1 gRNA and SCLT1-V5 Ultramer®) in the absence (DMSO) or presence (NU7441) of the DNA-PKcs inhibitor. (A) Centrioles are stained with anti-γTub antibody, V5 signal (if present) co-localizes with one of the two γTub-positive centrioles. White arrows indicate V5+ cells. Scale bar, 10 μm. Blow-ups without Hoechst 33342 are magnified 2.5×. (B) Bar chart showing the percentage of V5+ cells for each condition. N=3 independent experiments (with >100 cells visually scored for each condition), unpaired Student’s t test, ∗∗ = p < 0.05. (C) Electroporated RPE1, both untreated (DMSO) and treated with NU7441 were probed for V5 and compared to RPE1 parental cells. V5 signal, when present, corresponds to the expected molecular weight of 75 kDa.

Journal: STAR Protocols

Article Title: CRISPR/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells

doi: 10.1016/j.xpro.2021.100407

Figure Lengend Snippet: DNA-PKcs inhibition augments the efficiency of HDR-mediated gene editing Homozygous tagging of the centriolar distal appendage protein SCLT1 with the V5-epitope is presented as example. Characterization of the polyclonal population of RPE1 cells resulting from the electroporation with a SCLT1-V5-tagging RNP mixture (Cas9, SCLT1 gRNA and SCLT1-V5 Ultramer®) in the absence (DMSO) or presence (NU7441) of the DNA-PKcs inhibitor. (A) Centrioles are stained with anti-γTub antibody, V5 signal (if present) co-localizes with one of the two γTub-positive centrioles. White arrows indicate V5+ cells. Scale bar, 10 μm. Blow-ups without Hoechst 33342 are magnified 2.5×. (B) Bar chart showing the percentage of V5+ cells for each condition. N=3 independent experiments (with >100 cells visually scored for each condition), unpaired Student’s t test, ∗∗ = p < 0.05. (C) Electroporated RPE1, both untreated (DMSO) and treated with NU7441 were probed for V5 and compared to RPE1 parental cells. V5 signal, when present, corresponds to the expected molecular weight of 75 kDa.

Article Snippet: IDT™ Custom Alt-R® CRISPR-Cas9 guide RNA , N/A , https://eu.idtdna.com/site/order/designtool/index/CRISPR_CUSTOM.

Techniques: Inhibition, Electroporation, Staining, Molecular Weight

Journal: STAR Protocols

Article Title: CRISPR/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells

doi: 10.1016/j.xpro.2021.100407

Figure Lengend Snippet:

Article Snippet: IDT™ Custom Alt-R® CRISPR-Cas9 guide RNA , N/A , https://eu.idtdna.com/site/order/designtool/index/CRISPR_CUSTOM.

Techniques: Recombinant, Purification, Plasmid Preparation, CRISPR, Electroporation, Software

Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with Cas9 and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.

Journal: Fundamental Research

Article Title: CMKLR1 senses chemerin/resolvin E1 to control adipose thermogenesis and modulate metabolic homeostasis

doi: 10.1016/j.fmre.2022.06.014

Figure Lengend Snippet: Chemerin and RvE1 differentially regulated mTORC1 signaling via CMKLR1. (a) Gene set enrichment analysis (GSEA) analysis for gene signatures of mTOR signaling pathway in iWAT from Rarres2 KO compared with C57BL/6J control mice. NES, normalized enrichment score. FDR, false discovery rate. (b, c) Western-blot analysis for level of p-S6K and S6K protein in undifferentiated 3T3-L1 treated with 100 ng/ml chemerin (b) or 100 ng/ml RvE1 (c). (d, e) Western-blot analysis for level of UCP1, p-S6K and S6K protein in 3T3-L1 mature adipocytes treated with 1 µM CL, 1 µM CL with 100 ng/ml chemerin (CL+CHE), 10 µM Mi or 100 ng/ml (0.285 µM) RvE1. (f) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, chemerin-9 (1 µg/mouse/day), Rapamycin (2 mg/kg/day) or Rapamycin (2 mg/kg/day) with chemerin-9 (1 µg/mouse/day) over 7 days. Western-blot analysis for level of UCP1 protein in iWAT. (g) C57BL/6J mice fed with NCD for 8 weeks were injected with Vehicle, RvE1 (1 µg/mouse/2day), Rapamycin (2 mg/kg/2day) or Rapamycin (2 mg/kg/2day) with RvE1 (1 µg/mouse/2day) over 14 days. Western-blot analysis for level of UCP1 protein in iWAT. (h-i) Western-blot analysis for level of p-S6K, S6K, UCP1 and CMKLR1 protein in different 3T3-L1 cell pools treated with 100 ng/ml chemerin, 10 µM Mirabegron or 100 ng/ml RvE1 as well as transfected with Cas9 and guide RNA targeting the Cmklr1 gene sequence. gNS indicates 3T3-L1 cell pools transfected with Cas9 only and g#1 indicates 3T3-L1 cell pools transfected with Cas9 and guide RNA. The ImageJ software was used for gray scanning.

Article Snippet: The Cas9 lentivirus vector and gRNA1/2 lentivirus vector were purchased from Cyagen Biosciences and transduced to 3T3-L1 cells.

Techniques: Control, Western Blot, Injection, Transfection, Sequencing, Software